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LA RIGENERAZIONE AUTOLOGA POTENZIATA

La Rigenerazione Autologa Potenziata

Date: lunedì 24 febbraio

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LA RIGENERAZIONE AUTOLOGA POTENZIATA

Costo: • non soci C.A.M.I.G. -> • soci C.A.M.I.G ->

Programma:

•Clinical use of adult mesenchymal stem cells (MSCa) in medicine
•Enhancement strategies, including genetic manipulation, priming, and modification of culture method.

Location

live webinar

Orario: 21:15

Relatori: Paolo Mezzana -

Medico-Chirurgo, specialista in Chirurgia Plastica, Ricostruttiva ed Estetica, dottore di ricerca in Anatomia, Dermatologia e Chirurgia Plastica

Abstract: Photo-thermal biomodulation of microfiltered adult mesenchymal stem cells from
lipoaspirate: a new protocol for autologous exosomes release.
Mezzana P.
Background: Clinical use of adult mesenchymal stem cells (MSCa) in medicine
and regenerative surgery is constantly evolving. Adipose tissue-derived stem cells (ADSc) are
capable of inducing the production of new extracellular matrix (ECM) deposition of new collagen
and early revascularisation. However, poor engraftment, low survival rate of transplanted MSC,
and impaired donor-MSC potency under host age/disease result in unsatisfactory therapeutic
outcomes. Enhancement strategies, including genetic manipulation, priming, and modification of
culture method, have been investigated to generate highly functional MSC.
Photothermal biomodulation (PTBM) using simultaneously wavelengths from the red to near-
infrared light spectrum (620 and 850 nm), blue light (467nm) and a specific temperature (37°C)
induces mesenchymal stem cell activation and autologous exosomes release before the implant.
Methods: Disaggregated and microfiltered adipose tissue was prepared with Milligraft kit
(DualTrend, Italy). The ADSc are activated to release autologous exosomes with MCT® (Meta Cell
Technology, Spain) that is a system specially designed to ensure the effective and safe delivery of
photo thermal boost stimuli to biological tissues.
Results: Even after filtration of the disaggregated adipose tissue (AT), significant number of ADSc
was collected. Also in the non-filtered AT disaggregated highest number of cells was found, as
expected, but at the expense of more pronounced inflammation. Harvesting of AT with a 16
Gauge needle produces superior results compared to the cannula. The Authors demonstrate that
this method of ADSc purification allows the subsequent photo-thermal biomodulation and
autologous exosomes release with a standardized protocol and present 8 months clinical results.
Conclusions: With this new method in medicine and regenerative surgery it will be easier to
perform the isolation, purification and photo-thermal biomodulation of the ADSc as they are
isolated from the inflammatory component.
References:
Pinto H, Goñi Oliver P, Sánchez-Vizcaíno Mengual E. The Effect of Photobiomodulation on Human
Mesenchymal Cells: A Literature Review. Aesthetic Plast Surg. 2021;45(4):1826-1842.
Beltrán B, Sánchez MAR, Melamed G, Pinto H. Efficacy and safety of photothermal-bioactivated
platelet-rich plasma for facial rejuvenation. J Cosmet Dermatol. 2023;22(2):671-673.
Svolacchia F, Svolacchia L. Microfiltered vs only disaggregated mesenchymal stem cells from
adipose tissue in regenerative medicine. Scr Med 2020;51(3):152-157.
Li, M., Jiang, Y., Hou, Q. et al. Potential pre-activation strategies for improving therapeutic efficacy
of mesenchymal stem cells: current status and future prospects. Stem Cell Res Ther
2022;13:146-167.

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